Our Keyence BZ-X700 microscope is also capable of multi-day timelapse experiments. Note: we have enabled users to successfully do 8 day timelapse experiments on FV3000RS (Jawara Allen and Cindy Sears). Please also communicate with other users (typically through image core manager since users cannot see who has other reservations) in case the prior and/or next user is operating at 37 C. Similarly, if you can schedule consecutive days, then you could arrange with image core manager to keep the FV3000RS at 37 C overnight (usually at no charge to you), to avoid unnecessary temperature shifts. for example, much better one long session, 8-9am on, 9am-4pm expt, 4-5pm off, than a series of 1 hour on-expt-off sessions. Schedule your FV3000RS time to keep the incubator at 37 C. We prefer the doors be closed overnight - late afternoon users should discuss procedures with image core manager.īest practices: Plan ahead many days in advance for 37 C experiments. Note: we reserve the rightr to bill during the time for short sessions.ĭuring warm up, experiment, and cool down times, you need to cover all openings to the microscope stand (objective lenses and electronics) so humidity cannot damage the equipment.įor cool down, the openings to the objective lens and base need to stay covered, and usually the front and top doors ofthe plexiglas shroud will be opened to facilitate cool down. McNamara, we will typically change the session billing to make the 'up' and 'down' time no charge. The incubator requires 1 hour to warm up to stable temperature, and 1 hour to cool down to room temperature. The user needs to make their iLab reservation to include these two hours. If they communicate with the image core manager (Dr. The FV3000RS has an OkoLab incubator with temperature control (usually 37 C) and optional 5% CO2. FV3000RS Confocal Microscope Incubator Policy June 14, 2022: a web site limitation prevents us from adding more content to this web page - please see 'fv3000_part2'Īpril 19, 2022: Incubator control policy - (temperature and/or CO2) - (this policy has been in place since FV3000RS arrival 8/2018) adding on this web pagefor clarity. See below for more details, NIH RePORTER page is here. Please acknowledge NIH shared instrumentation grant 1S10OD025244-01 (Prof.

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